Jae-Young Cho

Jae-Young Cho

First Name: 

Jae-Young

Last Name: 

Cho

Email: 

jae-young.cho@nrc-cnrc.gc.ca

Poster Abstract: 

Address: 

11421 Saskatchewan Drive Edmonton, Alberta, T6G 2M9

Remote: 

Saskatoon

Abstract Title: 

Microscopic Characterization of Nanocarrier Complexes for Effective Microspore Transfection Protocol using SEM, TEM and AFM

Abstract Authors: 

Jae-Young Cho1, Usha Hemraz1, Pankaj Bhowmik2, Goska Nowak2 and Patricia Polowick2
1National Institute for Nanotechnology, National Research Council (NINT-NRC) 11421 Saskatchewan Drive, Edmonton, Alberta T6G 2M9, Canada
2NRC-Saskatoon, 110 Gymnasium Place, Saskatoon, Saskatchewan S7N 0W9, Canada E-mail: jae-young.cho@nrc-cnrc.gc.ca

Abstract Text: 

Intensive research on gene transfection tools using nanomaterials have been completed over the past several years as they show potential in biomedicine and therapeutic applications. However, there are many barriers to the successful use of these new nanomaterials, especially in plants. One hurdle is the development of an effective delivery system, and the production of nanocarrier complexes is the most critical factor.

There are many different nanocarriers for complexation, such as nanotubes and nanoparticles. Among these, rosette nanotubes (RNTs) have been brought to our attention since they are biocompatible nanomaterials generated from the self-assembly of a bio-inspired bicycle that features the hydrogen bonding arrays of guanine and cytosine.

In this study, we used these biocompatible self-assembled RNTs functionalized with oligo-lysine side chains. To find the most effective method for making suitable nanocarrier complexes, we focused on the interaction between RNTs and plasmid DNA under different conditions, imaging the details using scanning electron microscopy (SEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).