Bhavana Deore

Bhavana Deore

First Name: 

Bhavana

Last Name: 

Deore

Email: 

bhavana.deore@nrc-cnrc.gc.ca

Poster Abstract: 

Address: 

Security and Disruptive Technologies 100 Sussex Drive, Room 1047, Ottawa, ON, Canada

Remote: 

Saskatoon

Abstract Title: 

Wheat Improvement through Cell Technologies (WICT): The Development of DNA/Silica Nanoparticle Nanovectors for Transfection Studies in Plant Cells

Abstract Authors: 

Bhavana Deore1, Michael L. Barnes1, Arnold Kell1, Pankaj Bhowmik2, Patricia Polowick2; 1National research Council, Ottawa, ON Canada, K1A R06, 2National research Council, Saskatoon, SK Canada, S7N 0W9

Abstract Text: 

There are very interesting materials chemistry challenges in the development of nanovectors for wheat research because the cell walls and prominent exine in microspores makes endocytosis and subsequent delivery of protein/DNA cargo much more difficult than in mammalian cells. Silica nanocarriers are promising candidates due to their multifunctionality, stability, tunable size and charge and biocompatibility. In this project, our objective is to develop a series of multifunctional luminescent silica nanoparticles that are surface-modified with a variety of cationic ligands/cell penetrating peptides and subsequently use to immobilize gene editing tools/plasmid DNA/ gene cassettes for delivery to wheat microspores. The luminescence built into the nanoparticles provides the unique ability to track the uptake of the carriers into the microspores.
Here, we prepared complexes of plasmid DNA (pDNA, labeled and unlabeled) and silica nanoparticles (SNP) functionalized with amine crosslinked fluorescein isothiocyanate fluorophore (SNP-FITC-APTES) through electrostatic interaction. The resulting SNP/pDNA complexes were isolated/ purified and then the optimized ratios were used for transfection studies into wheat microspores. The preliminary results indicate the transfection of plasmid DNA into wheat microspores through designed SNP/pDNA nanovectors.