Intensive research on gene transfection tools using nanomaterials have been completed over the past several years as they show potential in biomedicine and therapeutic applications. However, there are many barriers to the successful use of these new nanomaterials, especially in plants. One hurdle is the development of an effective delivery system, and the production of nanocarrier complexes is the most critical factor.
There are many different nanocarriers for complexation, such as nanotubes and nanoparticles. Among these, rosette nanotubes (RNTs) have been brought to our attention since they are biocompatible nanomaterials generated from the self-assembly of a bio-inspired bicycle that features the hydrogen bonding arrays of guanine and cytosine.
In this study, we used these biocompatible self-assembled RNTs functionalized with oligo-lysine side chains. To find the most effective method for making suitable nanocarrier complexes, we focused on the interaction between RNTs and plasmid DNA under different conditions, imaging the details using scanning electron microscopy (SEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM).