Fusarium head blight (FHB) caused by Fusarium graminuearum (Fg) is one of the most prevalent diseases of wheat (Triticum aestivum L.) and other small grain cereals. It has proven difficult to move existing sources of FHB resistance into adapted Canadian varieties due to poor agronomics and low yield. Recently, a novel source of FHB resistance, FL62R1, which has high FHB resistance and high agronomics and yield, was created by germplasm developers at Agriculture and Agri-Food Canada. In this study, we will use genomic and molecular approaches to characterize this new FHB resistance and with the goal of eventually introgressing desirable alleles into Canadian elite wheat varieties. The spread of Fg in heads of FL62R1 was considerably reduced compared to susceptible varieties, and the high level of type II resistance observed was similar to the well-known FHB resistant variety, Sumai3. Fungal progression was monitored by using a GFP-tagged Fg strain. Microscopic data showed that Fg was effectively blocked in the rachis and did not spread to uninoculated spikelets of FL62R1. Double haploid mapping populations of FL62R1 crossed with two Canadian elite wheat varieties have been generated for genetic analysis.